25% Off Immunofluorescence Antibodies - Use Code: "LUCKYIF24" - Valid 3/15-3/22/24 for U.S. customers only
Protocols
Bioss is dedicated to helping you achieve exceptional results. Our detailed listings for reagnets and buffers for your application can be found below.



 



Reagents and Bufferspdf iconDOWNLOAD A PDF

Phosphate-Buffered Saline (PBS)(10x)
  • 80g of NaCl
  • 2.0g of KCl
  • 14.4g of Na2HPO4
  • 2.4g of KH2PO4
  • Mix 800mL ultra-pure water and adjust pH to 7.6 with pure HCl. Top up with ultra-pure water to 1L.
Phosphate-Buffered Saline w/ Tween20 (PBST)
  • For 1L: 100mL of PBS 10x + 890mL ultra-pure water + 10 mL Tween 20
Tris-Buffered Saline (TBS) (10X)
  • 24.23g Trizma HCl
  • 80.06g NaCl
  • Mix in 800mL ultra-pure water and adjust pH to 7.6 with pure HCl. Top up with ultra-pure water to 1L.
Tris-Buffered Saline w/ Tween20 (TBST)
  • For 1L: 100mL of TBS 10x + 890mL ultra-pure water + 10 mL Tween 20


Western Blotting Buffers

RIPA Buffer
  • 150mM NaCl
  • 1.0% NP-40 or 0.1% Triton X-100
  • 0.5% sodium deoxycholate
  • 0.1% SDS (sodium dodecyl sulphate)
  • 50mM Tris-HCl pH8.0
  • Protease Inhibitors
Tris-HCl buffer
  • 20mM Tris-HCl pH7.5
  • Protease Inhibitors
Denaturing lysis buffer
  • 1% SDS
  • 5mM EDTA
  • Immediately before use add:
    • 10mM dithiothreitol or beta-mercaptoethanol
    • Protease inhibitors
    • 15U/mL DNase1
Laemmli 2X buffer/loading buffer
  • 4%SDS
  • 10% 2-mercaptoethanol
  • 20% glycerol
  • 0.004% bromophenol blue
  • 0.125M Tris-HCl
  • Check the pH and adjust pH to 6.8.
Running buffer
  • 25mM Tris base
  • 190mM glycine
  • 0.1% SDS
  • Check the pH, which should be about pH 8.3. Adjust if necessary.
Transfer buffer (wet)
  • 25mM Tris base
  • 190mM glycine
  • 0.1% SDS
  • The pH should be about pH8.3. Adjust if necessary.
Transfer buffer (semi-dry)
  • 48mM Tris
  • 39mM glycine
  • 20% methanol
  • 0.04% SDS
Blocking buffer
  • 5% milk or BSA(bovine serum albumin)
  • Add to TBST buffer. Mix well and filter.

 



Immunohistochemistry/Immunocytochemistry Buffers

Formalin solution (10%)
  • 3.7-4% Formaldehyde (37-40%)
  • 33mM NaH2PO4
  • 46mM Na2HPO4
Paraformaldehyde (4%)
  • 8% paraformaldehyde
0.2M Phosphate Buffer(PB) pH7.4
  • 53mM NaH2PO4
  • 154mM Na2HPO4
  • Heat 8%PFA solution at 60C while stirring. Once the solution reaches 60C and the PFA dissolves, add 500mL of 0.2M phosphate buffer, to bring the solution to 4%PFA in 0.1M phosphate. Carefully add 1N NaOH until the solution is clear. Cool the solution and filter.
Sodium Citrate Buffer pH6.0
  • 10mM sodium citrate
  • 0.05% Tween20
  • Mix to dissolve sodium citrate and adjust pH to 6.0 with 1N HCl.
  • Add Tween20 and mix well.
  • Store at room temperature for 3 months or at 4C for longer storage.

 



ELISA Buffers

Bicarbonate/carbonate coating buffer (1oomM) pH9.6
  • Antigen or antibody should be diluted in coating buffer to immobilize them to the wells:
  • 29mM Na2CO3
  • 71mM NaHCO3
Blocking solution
  • Commonly used blocking agents are 1% BSA, serum, or non-fat dry milk in PBS.
Wash solution
  • Usually PBS or Tris-buffered saline(pH7.4) with detergent such as 0.05% (v/v) Tween 20 (TBST).

 



Flow Cytometry

FACS buffer/antibody dilution buffer
  • 10% FCS
  • 1% sodium azide in PBS
Permeabilization
  • 0.1-1% Triton X-100/NP-40 in PBS
Fixative
  • 0.01-0.1% paraformaldehyde in PBS.